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KMID : 0545120080180020334
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 2 p.334 ~ p.337
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Control of Acetate Production Rate in Escherichia coli by Regulating Expression of Single-Copy pta Using lacQ in Multicopy Plasmid
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Lee Sun-Gu
Liao James C
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Abstract
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A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosphate was constructed by integrating pta, a gene encoding phosphotransacetylase, under a tac promoter into the chromosome of E. coli with a ptanegative background and transforming a multicopy plasmid containing the lacIQ gene into the strain. The production rate of acetate was shown to be tightly controlled when varying the concentration of the inducer (IPTG) in the model system.
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KEYWORD
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Metabolic engineering, tight regulation, metabolic flux, tac promoter, lacI
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